2D-Cross Correlation Spectroscopy Coupled with Molecular Fluorescence Spectroscopy for Analysis of Molecular Structure Modification of Camel Milk and Cow Milk Mixtures during Coagulation

Oumayma Boukria , El Mestafa El Hadrami , Shaxnoza Sultanova , Jasur Safarov , Françoise Leriche (1) , Abderrahmane Ait Kaddour (1)

1 UMRF - Unité Mixte de Recherche sur le Fromage

Synchronous fluorescence spectroscopy (SFS) coupled with two-dimensional correlation spectroscopy (2DCOS) was employed to monitor, at the molecular level, the coagulation of five mixture ratios of camel's milk (CaM) and cow's milk (CM) (100% CaM, 75% CaM:25% CM, 50% CaM:50% CM, 25% CaM:75% CM and 100% CM). The dissimilarities among the different formulations are highlighted on the synchronous 2DCOS-SFS. In addition, according to the cross-peak symbols in synchronous and asynchronous spectra, the rate of response modification in riboflavin, protein and vitamin A matched with common coagulation phenomena usually reported during chymosin coagulation (hydrolysis of κ-casein, destabilization of casein micelles and aggregation). This study demonstrated that 2DCOS-SFS is a successful strategy to discriminate milk mixtures and to monitor molecular structure modifications during coagulation process.

Mots clés: milk; mixture; spectroscopy; 2D COS

Domaines: Sciences du Vivant [q-bio]

Oumayma Boukria, El Mestafa El Hadrami, Shaxnoza Sultanova, Jasur Safarov, Françoise Leriche, et al.. 2D-Cross Correlation Spectroscopy Coupled with Molecular Fluorescence Spectroscopy for Analysis of Molecular Structure Modification of Camel Milk and Cow Milk Mixtures during Coagulation. Foods, 2020, 9 (6), pp.724. ⟨10.3390/foods9060724⟩. ⟨hal-03081570⟩